Influence of high pressure on conformational changes of soybean glycinin

摘要

Influence of high pressure on conformational changes of soybean glycinin was studied by means of sulphydryl groups detection, spectrofluorimetry, ultraviolet difference spectra, circular dichroism, differential scanning calorimetry and electrophoresis. The results indicated that glycinin were dissociated into subunits and the conformation of these subunits had been changed after high pressure processing. More sulphydryl groups as well as hydrophobia regions and amino acid residues which had ultraviolet absorbance had been found significantly after high pressure processing (>= 300 MPa). DSC analysis results demonstrated that glycinin had been denatured completely after processed at 400 MPa for 10 min. The CD analysis indicated that some of the ordered structures of alpha-helix and beta-structure were destroyed and converted to random coil after processed at 500 MPa for 10 min.