Effective RNA Isolation for Today's Sensitive Analysis Methods

摘要

Genomic DNA contamination is one of the most common challenges facing researchers who isolate RNA. The complete separation of RNA and DNA is technically challenging because they share structural similarities, and RNA is subject to degradation by ribonucleases (RNases). Essentially, all existing methods for isolating total RNA copurify genomic DNA, which interferes with sensitive detection methods. A common approach to eliminate DNA contamination is to treat the RNA sample with DNase. However, this approach requires additional steps and is not always entirely effective. There is also a risk of losing valuable samples due to RNase contamination. Thus, a versatile method is needed to isolate pure total RNA that is free of contaminating DNA from a widevariety of sample types.