Production of polyclonal antibodies for detection of Nucleopolyhedrovirus infecting Helicoverpa armigera.

摘要

Polyclonal antibodies were raised against purified polyhedrin [polyocclusion body (POB)] protein preparations of Helicoverpa armigera nucleopolyhedrovirus (HaNPV) and were used to develop a direct antigen coating enzyme-linked immunosorbent assay (DAC-ELISA) to detect HaNPV. The sensitivity of the DAC-ELISA was 15 ng/ml of partially purified viral protein or 30 ng/ml POBs from the HaNPV infected larval extracts. The antibodies were highly specific to polyhedrin protein of HaNPV and were useful to diagnose NPV at early stages of larval infection and also for the quantification of the NPVs during production of viral insecticides.