Method for the detection of microcistinas and / or nodularina in environmental samples; diagnostic kit, polyclonal antibody for the detection of microcistinas and / or nodalarina in environmental samples and production method of the polyclonal antibody

摘要

Method for the detection of microcistinas and / or nodularina in environmental samples; diagnostic kit, polyclonal antibody for the detection of microcistinas and / or nodalarina in environmental samples and production method of the polyclonal antibody.The present invention relates to a method for detection of microcistinas and / or nodalarina in environmental samples comprising the steps of raising microtitation plates with the antigen MCLR; block the plates with a solution of casein 25%; to produce the formation of a imunocomplexo; add 100 109 L of imunocomplexo formed in step (III) the microtitre plate blocked; add conjugated anti IgG labeled with peroxidase and reveal the reaction with the substrate OPD. The present invention also relates to the production of a diagnostic kit for the detection of microcistinas and / or nodularina using that method.