Synthesis and fluorescence properties of 3-benzoxa- and thiazol-2-ylquinoline-5 or 7-maleimides

摘要

Synthesis of four new 3-benzoxa/thiazol-2-ylquinolinemaleimides and their fluorescence properties are described.Among these,the 3-benzothiazol-2-ylquinoline-5-maleimide shows very good enhancement in fluorescence upon thiol addition and thus would serve as a sensitive thiol detection and quantitation reagent.Similarly,3-benzoxazol-2-ylquinoline-7-maleimide forms a fluorescent conjugate with a model protein BSA that has good emission properties,both in the native and in the denatured state,as shown by fluorescence spectroscopy and SDS-PAGE experiments,thus indicating that 3-benzoxazol-2-ylquinoline-7-maleimide would be useful as a protein label that gives highly fluorescent protein bioconjugates.