Anti-influenza virus activity of tricin,4',5,7-trihydroxy-3',5'-dimethoxyflavone.

摘要

BACKGROUND: We examined the anti-influenza virus activity of tricin,4',5,7-trihydroxy-3',5'-dimethoxyflavone, against five viruses: A/Solomonislands/3/2006 (H1N1), A/Hiroshima/52/2005 (H3N2), A/California/07/2009(H1N1pdm), A/Narita/1/2009 (H1N1pdm) and B/Malaysia/2506/2004 strains in vitroand against A/PR/8/34 virus in vivo.METHODS: The effect of tricin was studied by an infectious virus yield reduction assay. The anti-influenza virus mechanism of the tricin was examined by westernblot analysis, real-time reverse transcriptase PCR analysis, haemagglutinationinhibition (HI) assay and neuraminidase (NA) inhibition assay. The anti-influenzavirus efficacy of tricin was further examined in a murine influenza virusinfection model.RESULTS: Tricin of 3.3 to 30 μM significantly reduced seasonal A (H1N1), (H3N2)viruses, novel A (H1N1pdm) virus, as well as B virus in a dose-dependent manner. The 50% effective concentrations of tricin were 3.4 μM for seasonal A (H3N2)virus, 4.9 μM for B virus and 8.2 μM for A/Narita (H1N1pdm) virus. Tricindecreased the expression of haemagglutinin (HA) protein and matrix (M) protein,and messenger RNA expression of HA and M of influenza virus in the infectedcells. Tricin exhibited little or no effects on influenza virus HI and NAactivities. In the mouse infection model, tricin was significantly effective inreducing body weight loss, and also effective in prolonging survival times ofinfected mice.CONCLUSIONS: Tricin was indicated to possess anti-influenza virus activity and toameliorate body weight loss and survival rate of influenza-A-virus-infected mice.Tricin is a novel compound with potential anti-influenza virus activity in vitro and in vivo.