Immunogenicity and protective ability of RpoE againstStreptococcus suisserotype 2

摘要

Aims RpoE is quite immunogenic and can be used as a candidate vaccine forStreptococcus suisinfection via immunoproteomics as reported in our previous studies. In this study, we aimed to verify the immunogenicity of recombinant RpoE and its protective effect against ofS. suis. Methods and Results The RpoE protein was successfully expressed inEscherichia coli, and the purified recombinant protein was mixed with ISA206 to prepare anS. suissubunit vaccine. Mice were immunized with the RpoE subunit vaccine and then infected with the virulentS. suisstrain ZY05719. Subunit vaccine-immunized mice achieved 50% protection, less pathological damage and less bacterial distribution in each organ compared with the control mice. Furthermore,in vitroculture, showed that mouse antisera significantly (P < 0 center dot 001) inhibited the growth ofS. suis, and qRT-PCR results showed that RpoE successfully induced the up-regulation of IL-6 and TNF-alpha cytokines. Conclusions RpoE mice were vaccinated to obtain immune protection, which may be candidates forS. suissubunit vaccine. Significance and Impact of the Study The results of this study will provide new ideas for the development of safe and effective recombinant subunits vaccines forS. suis.