Extraction optimization of antifungal compounds from Thalictrum foliolosum DC. roots

摘要

Thalictrum foliolosum DC. is a perennial herb of the Ranunculaceae family that has been used to cure high fever, inflammation, stomachache, and malaria in traditional Chinese medicines for centuries. The present study aims at the optimization of a hydro-alcoholic ultrasound-assisted extraction (UAE) for antifungal extracts from dry T. foliolosum roots (TFr) in vitro and their antifungal activity against Penicillium italicum by applying response surface methodology (RSM). Based on a Box-Behnken design (BBD), the obtained results Results showed that the suitable conditions of UAE method were as follows: extraction time of 70 min, ethanol concentration of 55%, liquid-to-solid ratio of 34 mL/g and extraction temperature of 75 degrees C. Under these conditions,the experimental inhibitory zone (59.33 +/- 0.55 mm diameter) of TFr extract against P. italicum was well-matched with the predicted value (59.46 mm). The mycelial growth assay showed that TFr extract possessed a broad antifungal spectrum against thirteen phytopathogens. Both in vitro and in situ antifungal assays indicated that TFr extract as a natural antifungal agent can be potentially applied to control postharvest blue mold disease in citrus fruits. (c) 2021 SAAB. Published by Elsevier B.V. All rights reserved.