Optimized extraction of limonene and its characterization as well as antifungal agent against emerging yeasts.

摘要

Optimized extraction of limonene and its characterization as well as antifungal agent against emerging yeasts this patent deals with a method of extraction optimized limonene from bark of homeAnja and its subsequent application as antifungal against planktonic cells and biofilmes emerging clinical yeast.The extraction of citrus base was obtained by steam distillation of drag by fractionated.In vitro determination of minimum inhibitory concentration (MIC) of the extracts against emerging strains of genus Candida (condida GUI. Liermondii, C. haemulonii, C. lusitaniae, C. pelliculosa, C.Famata and C. Ciferri) was carried out based on the method of micro dilution broth in the clinical laboratory standard institute cl51. The yield of extraction was 1 to 2 ml every 50 - 60g of the shell.The limonene presented MIC values of 4 - 16u00b5 g.ml-lfrente planktonic cells and biofilm.The results were higher than the reference antifungal amphotericin B and fluconazole had greater inhibitory concentrations ( 1 u00b5 g.ml-1).These results allow to glimpse the possibility of an extraction more efficient limonene, besides the product become a future alternative for the treatment of invasive candidiasis.