Optimization of supercritical CO 2 extraction and characterization of antifungal activity of essential oils in Cuminum cyminum L.

摘要

Supercritical CO 2 extraction of essential oils from Cuminum cyminum seeds was optimized by the adjustment of pressure, particle size, CO 2 flux and extraction temperature. An orthogonal test of L 16 (4 5 ) was designed to obtain an optimized combination of these four factors. Result showed that extractions performed at 34.5 MPa and 30°C using a particle size 60-80 mesh and a CO 2 flux of 80 mL, the extraction ratio for C. cyminum seed essential oil was 21.59%, which was higher than the ratio for other common extraction methods. Furthermore, antifungal tests using 100 mg/mL of essential oil obtained using the optimized extraction method, showed that the oil can completely inhibit the mycelial growth of Sclerotinia sclerotiorum, and that the extraction ratio was positively correlated with the inhibitory effect of the oil against S. sclerotiorum (R 2 = 0.9777 ). The results of GC-MS analysis showed that the main component of the essential oil with the highest activity was 4-(1-methylethyl)-benzaldehyde (30.56%).