Comparative fingerprint and extraction yield of Diospyrus ferrea (willd.) Bakh. root with phenol compounds (gallic acid), as determined by uv-vis and ft-ir spectroscopy

摘要

Objective: To analyze the comparative finger print and extraction yield of D.ferrea root with phenol compound (Gallic acid), as determined by UV-Vis spectroscopy and FTIR spectroscopy.Method:The UV Vis spectroscopy and FTIR spectroscopy are adequate techniques to fingerprint comparatively and to evaluate the extraction yield of D.ferrea root extract. The higher extraction yield was recorded in ethanol comparatively superior and richer in phenol (gallic acid). Gallic acid has therapeutic application for inflammatory allergic diseases due to its ability to inhibit histamine. Finger print region was recorded between 500-3500 cm-1 for each extract and functional groups were identified and compared with the standard. Result: The extraction factor was superior in ethanol (270 nm) rich in polar molecules. The FTIR signal at 900, 1500, 1714, 3000, 3100cm-1 considered as a good indicator of phenol (gallic acid).The functional groups of each extract were identified.Conclusion: The UV and FTIR method was validated as a good tool to investigate the finger print and to predict the composition of different root extract of D.ferrea.