Development of a matrix solid phase dispersion-high performance liquid chromatography-tandem mass spectrometric method for multiresidue analysis of 25 synthetic colorants in meat products

摘要

A novel high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method is developed for multiresidue analysis of twenty five synthetic colorants in meat matrices with matrix solid phase dispersion (MSPD), Good linearity was obtained with a correlation coefficient of 0.9935-0.9999. The quantitation limits (LOQs) for 12 acidic colorants were in the range from 0.89 μg kg~(-1) (acid orange 2) to 50.4 μg kg~(-1) (ponceau 4R) except for tartrazine, and the LOQs for 13 basic colorants were in the range from 0.52 μg kg~(-1) (Rhodamine B) to 4.19 μg kg~(-1) (disperse blue 7); among them the LOO. for 4 triphenylmethane colorants was in the range of 0.77-0.96 μg kg~(-1) which is better than the minimum required performance limit (MRPL) of 2 μg kg~(-1) for the sum of malachite green and leucomalachite green. The average recoveries for all analytes at three spiked levels were in the range of 75.4-123% except for Rhodamine B, (120-130%), with a RSD of <11%. The results showed that the content of 8 allowable food colorants and 17 banned synthetic colorants was lower than their LOQ level. This MSPD-HPLC-MS/MS method has the advantages of being rapid, sensitive, accurate, and high-throughput, and can be applied for multiresidue analysis of synthetic colorants in meat products.