首页> 外文期刊>Analytical and bioanalytical chemistry >Qualitative and quantitative analysis of poly(amidoamine) dendrimers in an aqueous matrix by liquid chromatography-electrospray ionization-hybrid quadrupole/time-of-flight mass spectrometry (LC-ESI-QTOF-MS)
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Qualitative and quantitative analysis of poly(amidoamine) dendrimers in an aqueous matrix by liquid chromatography-electrospray ionization-hybrid quadrupole/time-of-flight mass spectrometry (LC-ESI-QTOF-MS)

机译:液相色谱-电喷雾电离-混合四极杆/飞行时间质谱(LC-ESI-QTOF-MS)定性和定量分析水性基质中的聚(胺基胺)树状聚合物

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摘要

This work introduces a liquid chromatography-electrospray ionization-hybrid quadrupole/time-of-flight mass spectrometry (LC-ESI-QTOF-MS)-based method for qualitative and quantitative analysis of poly(amidoamine) (PAMAM) dendrimers of generations 0 to 3 in an aqueous matrix. The multiple charging of PAMAM dendrimers generated by means of ESI has provided key advantages in dendrimer identification by assignation of charge state through high resolution of isotopic clusters. Isotopic distribution in function of abundance of isotopes ~(12)C and ~(13)C yielded valuable and complementarity data for confident characterization. A mass accuracy below 3.8 ppm for the most abundant isotopes (diagnostic ions) provided unambiguous identification of PAMAM dendrimers. Validation of the LC-ESI-QTOF-MS method and matrix effect evaluation enabled reliable and reproducible quantification. The validation parameters, limits of quantification in the range of 0.012 to 1.73 μM, depending on the generation, good linear range (R > 0.996), repeatability (RSD < 13.4 %), and reproducibility (RSD < 10.9 %) demonstrated the suitability of the method for the quantification of dendrimers in aqueous matrices (water and wastewater). The added selectivity, achieved by multicharge phenomena, represents a clear advantage in screening aqueous mixtures due to the fact that the matrix had no significant effect on ionization, with what is evidenced by an absence of sensitivity loss in most generations of PAMAM dendrimers.[Figure not available: see fulltext.]
机译:这项工作介绍了一种基于液相色谱-电喷雾电离-混合四极杆/飞行时间质谱(LC-ESI-QTOF-MS)的方法,用于定性和定量分析第0至9代的聚(酰胺基胺)(PAMAM)树状聚合物3在水性基质中。通过ESI生成的PAMAM树状聚合物的多次充电通过高分辨率同位素簇的电荷状态分配,为树状聚合物的鉴定提供了关键优势。同位素〜(12)C和〜(13)C的丰度函数中的同位素分布产生了有价值的和互补的数据,可用于可靠的表征。最丰富的同位素(诊断离子)的质量准确度低于3.8 ppm,可准确识别PAMAM树状聚合物。 LC-ESI-QTOF-MS方法的验证和基质效应评估可实现可靠且可重复的定量。验证参数,在0.012至1.73μM范围内的定量限(取决于代),良好的线性范围(R> 0.996),重复性(RSD <13.4%)和重现性(RSD <10.9%)证明了适用于水性基质(水和废水)中树枝状聚合物的定量方法。由于基质对电离没有显着影响,通过多电荷现象获得的增加的选择性代表了筛选水性混合物的明显优势,这在大多数世代的PAMAM树状聚合物中都没有灵敏度的降低。不可用:请参阅全文。]

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