Enzyme-Linked Immunosorbent Assay for the Determination of Total Ginsenosides in Ginseng

摘要

Hybridoma secreting a monoclonal antibody (MAb) against ginsenosides was produced by fusing splenocytes from a mouse immunized against a ginsenoside Re-bovine serum albumin (G-Re-BSA) conjugate with myeloma cell line SP2/0-Agl4.A MAb-4G10 had a wide cross-reaction with 20(S)-protopanaxadiol and 20(S)-protopa-naxatriol type ginsenosides.An enzyme-linked immunosorbent assay (ELISA) that had an effective measuring range of 20-400 ng/mL for total ginsenosides when G-Re was used as a standard.Total ginsenosides concentration in ginseng were determined by ELISA and high-performance liquid chromatography (HPLC) in a good agreement together,and the least-squares fit had a coefficient of determination (gamma~2) of 0.995.