Thin-Film Glucose Biosensor Based on Plasma-Polymerized Film: Simple Design for Mass Production

摘要

We propose a simple thin-film glucose biosensor based on a plasma-polymerized film.The film is deposited directly onto the substrate under dry conditions.The resulting films are extreme thin,adhere well onto the substrate (electrode),and have a highly cross-linked groups,which enable a large amount of enzyme to be immobilized.Since this design allows fabrication through a dry process,with the exception of the enzyme immobilization,which is the last stage of the process,the chip fabrication can be designed as a full-wafer process to achieve mass produced using this film are more reproducible,exhibit lower noise,and reduce the effect of interference to a greater degree than sensors made using conventional immobilization methods,e.g.,via 3-(aminopropyl)triethoxysilane.The obtained film is a good interfacial design between ensyme and electrode;enzyme two-dimensiomally locates very close to the electrode in a manner that is quite reproducible.Therefore,a wide dynamic range (up to 60 mM) and raid response time (11.5 +- 0.8 s) were obtained.Because of its highly cross-linking network structure,the amperometric response due to interferences such as ascorbic acid and acetaminophen was reduced by size discrimination of plasma-polymerized films.