A Highly Selective and Sensitive Chemiluminescent Probe for Real-Time Monitoring of Hydrogen Peroxide in Cells and Animals

摘要

Selective and sensitive molecular probes for hydrogen peroxide (H2O2), which plays diverse roles in oxidative stress and redox signaling, are urgently needed to investigate the physiological and pathological effects of H2O2. A lack of reliable tools for in vivo imaging has hampered the development of H(2)O(2)mediated therapeutics. By combining a specific tandem Payne/Dakin reaction with a chemiluminescent scaffold,H2O2-CL-510was developed as a highly selective and sensitive probe for detection of H(2)O(2)both in vitro and in vivo. A rapid 430-fold enhancement of chemiluminescence was triggered directly by H(2)O(2)without any laser excitation. Arsenic trioxide induced oxidative damage in leukemia was successfully detected. In particular, cerebral ischemia-reperfusion injury-induced H(2)O(2)fluxes were visualized in rat brains usingH(2)O(2)-CL-510, providing a new chemical tool for real-time monitoring of H(2)O(2)dynamics in living animals.