Degradation mechanism of Saccharomyces cerevisiae beta-D-glucan by ionic liquid and dynamic high pressure microfluidization

摘要

The insolubility of Saccharomyces cerevisiae beta-D-glucan in most common solvents seriously restricts its applications and recovery. In this work, ionic liquids (ILs) were used for dissolution and modification under high pressure microfluidization (HPM). ILs 1-ethyl-3-methylimidazolium acetate (EmimAc) showed good beta-D-glucan dissolving capacities, which can facilitate its structural modifications to recycle under environmentally friendly conditions. However, the synergistic effect of ionic liquid and high pressure microfluidization on physiochemical properties and structure of S. cerevisiae beta-D-glucan were unclearly. The objectives of this study were: 1) to study the physiochemical properties of beta-D-glucan after treating by ILs and HPM; 2) to compare the essential structure and conformation of beta-D-glucan after treating by ILs and HPM; 3) to provide theoretical basis for precise regulation and green manufacturing of soluble S. cerevisiae beta-D-glucan.