Synthesis of Artificial Nucleic Acids Functionalized by Fixation of Conformation

摘要

Recent studies of the synthesis of oligonucleotides incorporating conformationally locked nucleoside or nucleotide building blocks have been comprehensively reviewed in connection with the antisense stratagy as well as creaction of new functions of DNA/RNA. The synthesis, and conformational properties of a wide varaiety of nucleoside derivatives locked by introduction of three, four, and five membered-ring systems to the deoxyribose or ribose moiety have been described. The hybridization abilities of oligonucleotides incorporating these locked-nucleosides are also discussed. In addition, this review deals with the synthesis and properties of conformationally fixed cyclouridylate derivatives bridged between the uracil and the 5'-phosphate of 5'-uridylic acids and their successful application to the construction of bent DNA and RNA.