The Renal Effects of Vanadate Exposure! Potential Biomarkers and Oxidative Stress as a Mechanism of Functional Renal Disorders-Preliminary Studies

摘要

The alterations in the levels/activities of selected biomarkers for detecting kidney toxicity and in the levels of some oxidative stress (OS) markers and elements were studied in male rats to evaluate biochemically the degree of kidney damage, investigate the role of OS in the mechanism of functional renal disorders, reveal potential biomarkers of renal function, and assess the renal mineral changes in the conditions of a 12-week sodium metavanadate (SMV, 0.125 mgV/mL) exposure. The results showed that OS is involved in the mechanism underlying the development of SMV-induced functional renal disturbances. They also suggest that the urinary cystatin C (CysC_u) and kidney injury molecule-1 (KIM-1_u) could be the most appropriate to evaluate renal function at the conditions of SMV intoxication when the fluid intake, excreted urinary volume (EUV), body weight (BW), and the urinary creatinine excretion (Cre_u) decreased. The use of such tests as the urinary lactate dehydrogenase, alkaline phosphatase, gamma-glutamyltranspeptidase, and N-acetyl-beta-D-glucosaminidase (LDH_u, ALP_u, GGTP_u, and NAG_u) seems not to be valid given their reduced activities. The use of only traditional biomarkers of renal function in these conditions may, in turn, be insufficient because their alterations are greatly influenced by the changes in the fluid intake and/or BW.