リンバ球のバッグ培養における客観的細胞画像取得技術の開発

摘要

Evaluation of the cell culture process in activated autologous lymphocyte therapy is conducted by microscopic observation, and is entrusted to cell culture engineers. Because of the changes in medium permeation rate, color phase of medium, and cell shape and size during the course of culture, culture engineers must subjectively determine the optimal brightness and focus position when capturing cell images. In this study, an objective image capturing method was developed for bag culture of lymphocytes. Images of single cells and cell aggregates were captured at different levels of LED brightness, which was varied by changing the LED current. The color model used for the cell images was converted to the HSL color model（H: hue ; S: saturation ; L: lightness). The LED brightness was optimized automatically using the index (I) = (255 - L) x (L + S),and the focus position was selected to correspond to the position where the maximum number of particles was calculated for each cell image. In conclusion, using our image capturing method, it was possible to objectively capture cell images in bag culture of lymphocytes.