The Role of Supplement EF-2001 Containing Enterococcus faecalis on Murine Ileal Immune Responses Ex Vivo

摘要

The intestinal flora and Peyer's patches (PP) play an important role in increasing immunity, and ingestion of lactic acid bacteria is considered beneficial. We therefore studied how Enterococcus faecalis EF-2001 effects on the regulation of intestinal immunity, by using mouse PP cells with or without concanavalin A (Co A). Production of the T helper 1 (Thl), cytokine IL-2 in cultured IL-2 in culture PP cells co-stimulated with Con A was significantly decreased in mice administered with EF-2001 at dose of 300 mg/kg/day for five consecutive days. In addition, the production of interferon (IFN)-y, also attributed to Th 1 cytokines, was dramatically enhanced by EF-2001 administration at the same doses. However, EF-2001 did not alter the production of the T helper 2 (Th 2) cytokines such as IL-4 and IL-5 in the same treatment. Further, PP cells from mice administered with EF-2001 significantly produced B cell immunoglobulins, IgGl and IgA, co-stimulated with lipopolysaccharide LPS. Taken together, we suggested the possibility that EF-2001 modulates a gut immune response especially on B cell and Th 1, and contributes to maintaining homeostasis.