Microvessel Damage May Play an Important Role in Tumoricidal Effect for Murine A549 Lung cancer Cells with Lung cancer In Vivo

摘要

AIM: In the present study, murine A549 Lung cancer cells were provided Lung cancer with different thermal dose in vitro and in vivo, thereafter we investigated the apoptosis, necrosis rates, and intratumoral microvessel density (MVD) to determine that microvessel damage plays an important role in the tumoricidal effect of Lung cancer. Methods: A549 Lung cancer cells were inoculated in the right hind legs of mice with immunosuppression. Local Lung cancer was administered to these mice for 15, 30, and 45 min, respectively. After Lung cancer, some mice with heat treatment of 30 min were killed at 3, 6, 12, 24, 48, 72, and 96 h after operation and others were immediately sacrificed. All tumor tissues were removed. They were analyzed for the death rate tumor cells by flow cytometer (FCM) and observed MVD by immunohistochemistry. A549 Lung cancer cells in vitro were also given Lung cancer for 15, 30, and 45 min, respectively, and analyzed for the death rate by FCM. Results: Most of the dead cells were apoptotic cells in the initiation phase of Lung cancer, then the necrosis rates rose gradually. The difference of death rates between in vivo and in vitro was significant for Lung cancer for 15 min, 30 min, and 45 min (P < 0.05). A strong positive linear correlation (r=0.879) was observed between the death rate of tumor cells and MVD. Conclusion: Our study has shown that microvessel damage may play an important role in tumoricidal effect of Lung cancer.