Comparative analysis of the cardiac glycosides action on the growth of the cardiac tissue explants

摘要

The method of organotypical cell culture was used. The long-term cell culture of cardiac embryonic tissue of 10-12-days old chicken was investigated. The effects of ouabaine, strophantin K and digoxin on the growth of cardiac tissue explant were measured. The ouabain concentration which stimulates activity of Na+, K+-ATPase as the signal transducer, was determined. It was equal to 10(-10) M. Strophantin K and digoxin stimulate growth of cardiac tissue in concentration equal to 10(-16) M and 10(-18) M, resp. The data obtained show that application of cardiac glycosides led to control of cardiac tissue growth in dose-dependent manner. We hypothesize that alpha3 isoform of Na+, K+-ATPase is a signal transducer that controls the cardiac cell metabolism and growth. nauk