ANALYSIS OF CARDIAC GLYCOSIDE BY IMMUNOASSAY

摘要

PURPOSE:To allow the exact and simple discrimination and identification of respective cardiac glycosides even if the cardiac glycosides resembling each other coexist in a sample by developing the sample with thin-layer chromatography or paper chromatography, then detecting the cardiac glycosides by immunoassay. CONSTITUTION:The cardiac glycoside-contg. sample is first developed by the thin-layer chromatography or paper chromatography and thereafter, the sample is brought into contact with the nitrocellulose film immersed into a soln., such as physiological salt soln., contg. a crosslinking agent and the developed pattern is transferred and immobilized to the nitrocellulose film. The immunoassay is thereafter executed on the nitrocellulose film. If the cardiac glycosides resembling each other coexist in the sample, the antibodies used make a crossreaction with these compds. and the spots of both may be detected, but these spots have a difference in the mobility by the chromatography and since such difference is transferred as it is to the nitrocellulose film, the respective discrimination of these cardiac glycosides is possible and the identification thereof is possible as well.