Screening and isolation of cyclooxygenase‐2 inhibitors from the stem bark of        <i >Phellodendron amurensePhellodendron amurense        Ruprecht by ultrafiltration with liquid chromatography and tandem mass spectrometry, and complex chromatography

Li Sainan; Zhang Yuchi; Shi Dongfang; Hou Wanchao; Xia Jianli; Liu Chunming

摘要

Abstract > Nonsteroidal anti‐inflammatory drugs appear to reduce the risk of developing cancer. One mechanism through which nonsteroidal anti‐inflammatory drugs act to prevent carcinogenesis is inhibition of the activity of the enzyme cyclooxygenase‐2. The cyclooxygenase‐2 inhibitors are widely used to reduce the risk of developing cancer. Natural products are considered to be a promising source of several novel cyclooxygenase‐2 inhibitors. Ultrafiltration with liquid chromatography and mass spectrometry is an efficient method that can be applied to rapidly screen and identify the ligands from the barks of Phellodendron amurense Ruprecht. A continuous online method comprised of pressurized liquid extraction, countercurrent chromatography, and semi‐preparative liquid chromatography was developed for the efficient scaled‐up production of eight compounds with high purities. The bioactivities of the separated compounds were assessed by an in vitro enzyme inhibition assay. The use of bioactivity screening method combined with preparation method of bioactive compounds and an in vitro enzyme inhibition assay facilitated the efficient screening and isolation of the cyclooxygenase‐2 inhibitors from complex samples. This could be used as an efficient method for the large‐scale production of functional ingredients. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“main”XML：Lang =“en”> <标题类型=“main”>摘要</标题> >非甾体类抗炎药似乎降低了发展癌症的风险。非甾体抗炎药物的一种机制来防止致癌作用是抑制酶环氧化酶-2的活性。环氧氧酶-2抑制剂广泛用于降低发展癌症的风险。天然产物被认为是几种新型环氧化酶-2抑制剂的有前途的来源。具有液相色谱和质谱的超滤是一种有效的方法，可以施加到快速筛选并鉴定来自培养皿的吠声的吠声的甲克的配体</ i> Ruprocht。由加压液萃取，逆流色谱和半制剂液相色谱组成的连续在线方法，为高纯度的8种化合物的有效缩小生产而开发。通过体外酶抑制测定评估分离的化合物的生物活性。生物活性筛选方法结合生物活性化合物的制备方法和体外酶抑制测定的用途促进了复杂样品中环氧氧酶-2抑制剂的有效筛选和分离。这可以用作函数成分大规模生产的有效方法。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-35800/'>《Journal of separation science.》</a> <b style="margin: 0 2px;">|</b><span>2019年第10期</span><b style="margin: 0 2px;">|</b><span>共10页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Li Sainan&option=202" target="_blank" rel="nofollow">Li Sainan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Yuchi&option=202" target="_blank" rel="nofollow">Zhang Yuchi;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Shi Dongfang&option=202" target="_blank" rel="nofollow">Shi Dongfang;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hou Wanchao&option=202" target="_blank" rel="nofollow">Hou Wanchao;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Xia Jianli&option=202" target="_blank" rel="nofollow">Xia Jianli;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Liu Chunming&option=202" target="_blank" rel="nofollow">Liu Chunming;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Central LaboratoryChangchun Normal UniversityChangchun P. R. China;</p> <p>Central LaboratoryChangchun Normal UniversityChangchun P. R. China;</p> <p>Central LaboratoryChangchun Normal UniversityChangchun P. R. China;</p> <p>Central LaboratoryChangchun Normal UniversityChangchun P. R. China;</p> <p>Central LaboratoryChangchun Normal UniversityChangchun P. R. China;</p> <p>Central LaboratoryChangchun Normal UniversityChangchun P. R. China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/159.html" title="化学">化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Countercurrent chromatography&option=203" rel="nofollow">Countercurrent chromatography;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=cyclooxygenase‐2 inhibitors&option=203" rel="nofollow">cyclooxygenase‐2 inhibitors;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=pressurized liquid extraction&option=203" rel="nofollow">pressurized liquid extraction;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=semi‐preparative scale&option=203" rel="nofollow">semi‐preparative scale;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=traditional Chinese medicine&option=203" rel="nofollow">traditional Chinese medicine;</a> </p> <div class="translation"> 机译：逆流色谱;环加氧基酶-2抑制剂;加压液体萃取;半制备规模;中医; 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Screening and isolation of cyclooxygenase‐2 inhibitors from the stem bark of Phellodendron amurensePhellodendron amurense Ruprecht by ultrafiltration with liquid chromatography and tandem mass spectrometry, and complex chromatography

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