Isolation and Sequences Analysis of the alpa-gliadin Genes from Aegilops sharonensis

摘要

In this study, PGR primers were designed base on the known genes in wheat and its relatives to isolate a-gliadin genes from Ae. sharonensis, one species of the Sitopsis section of the genus Aegilops, which was traditionally considered as the B-genomedonor of tetraploid and hexaploid wheat. Three novel alpha-ghadin genes were obtained. Analyses of the nucleotide and deduced amino acids of the obtained genes indicated that they shared the high sequence identities and similar primary structures to theknovm a-ghadin genes. Further more, the extensive variations were found. A phylogenic analysis based on the multigene alignment of the deduced amino acid sequences showed that the a-ghadin genes derived from Ae. sharonensis and Ae. speltoides were significantly distinguished from those of diploid A and D genome progenitors of wheat, but clustered closed to some genes from tetra- and hexaploid wheats. Moreover, genes derived from Ae. sharonensis were more closed to wheat than from Ae. speltoides. This result suggested that the origin and formation of wheat B genome might be polyphyletic.