Hepatoprotective Effect of Ethyl Acetate Extract of Vitex doniana Stem Bark on Carbon Tetrachloride (CC14) Induced Liver Damage in Wistar Rats

摘要

The hep atop rotective effect of Ethyl Acetate Extract of Vitex doniana (EAEVD) stem bark on carbon tetrachloride (CC14) induced hepatic damage was studied, to evaluate some biochemical parameters, to determine the in vitro antioxidant effect of the extract on Superoxide Dismutase (SOD), Catalase (CAT) glutathione peroxidase (GPX) and glutathione-s-transferase (GST) and to quantify the levels of some phytochemicals present in EAEVD stem bark. A total of twenty four Rats were used for the study. Animals in group 1 served as vehicle control, Group 2 served as hepatotoxin (CCL_4) treated group, Group 3 served as positive control (Sylimarin) treated group, Group 4 was administered with 100 (mg kg~(-1) b.wt.) of the extract and group 5 was administered with 200 (mg kg~(-1) b.wt.) of the extract After the experimental period of 14 days. The animals were sacrificed, blood and liver samples were collected and used for the evaluation of the following biochemical parameters Aspartate amino transferase (AST),Alanine amino transaminase (ALT) and Alkaline phosphatase (ALP) in serum, as well as thiobarbituric acidreactive substances (TBARS), lipidhydroperoxides superoxide dismutase (SOD), catalase (CAT) glutathione peroxidase (GPX) and glutathione-s-transferase (GST) in the liver. Administration of 100 and 200 mg kg~(-1) b.wt. (EAEVD) significantly decreased (p<0.05) AST, ALT, ALP TBARS and lipid hydroperoxides with a significant increase (p<0.05) in the levels of SOD, CAT, GPX and GST in in group 4 and 5. Twenty five mg kg~(-1) b.wt. sylimar in was used as standard, The results show that the oral administration of EAEVD plant prevents the progression of hepatic damage in Ccl_4 treated wistar albino rats and suggest that the extract could be effective in themanagement of liver problems.