首页> 外文期刊>Journal of Food Composition and Analysis >Profiling of triacylglycerol composition in arachidonic acid single cell oil from Mortierella alpina by using ultra-performance liquid chromatography-electrospray ionization-quadrupole-time-of-flight mass spectrometry
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Profiling of triacylglycerol composition in arachidonic acid single cell oil from Mortierella alpina by using ultra-performance liquid chromatography-electrospray ionization-quadrupole-time-of-flight mass spectrometry

机译:通过使用超级性能液相色谱 - 电喷雾电离 - 四极轴 - 飞行时间质谱法从Mistierella Alpina在花生素酸单细胞中谱分析。

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摘要

Despite the increasing interest in arachidonic acid single-cell oil (ARA-SCO), the information about its triacylglycerol (TAG) composition still limited, keeping in mind that the profiling of TAG composition considered a challenging task due to the complexity of its structure. For tackling such a task, a comprehensive characterization of TAG molecular species in two different samples of ARA-SCO from Mortierella alpina was performed by using ultra-performance liquid chromatography with quadrupole-time-of-flight mass spectrometry (UPLC-QTOF-MS). In the first sample, a total of 77 different TAGs were identified, while 73 TAGs were identified in the other sample. The most abundant TAG species in the first sample were (20:4-20:4-20:4), (20:3-20:4-16:0), (20:416:0-20:4), (18:1-16:0-20:3) and (24:0-20:3-18:1) with a relative content of 13.36, 8.43, 6.63, 4.52 and 4.27%, respectively. While (20:4-20:4-20:4), (20:4-24:0-20:4), (20:3-20:4-16:0), (20:4-16:0-20:4) and (18:1-16:0-20:3) were the major species in the second sample with a relative content of 11.47, 8.65, 7.84, 6.34 and 4.31%, respectively. Moreover, arachidonic acid, lignoceric acid, and palmitic acid were the predominant fatty acids in both ARA-SCO samples. This is the first work that reported an extensive characterization of TAG fractions of ARA-SCO, which might represent the key point for further studies and applications in the field of structured lipids and lipidomics.
机译:尽管对花生酸单细胞(ARA-SCO)的兴趣日益越来越令人兴趣,但其TRIOCYLGLYCEROL(标签)组成的信息仍然有限,请记住,由于其结构的复杂性,标签组合物的分析被认为是一个具有挑战性的任务。为了解决这种任务,通过使用超级性液相色谱法使用超级液相色谱法(UPLC-QTOF-MS)的超级性能液相色谱(UPLC-QTOF-MS),对来自Mortierella Alpina的两种不同样品的标签分子种类的综合表征。 。在第一样品中,鉴定了总共77种不同的标签,而在其他样品中鉴定出73个标签。第一个样品中最丰富的标签物种(20:4-20:4-20:4),(20:3-20:4-16:0),(20:416:0-20:4), (18:1-16:0-20:3)和(24:0-20:3-18:1),相对含量分别为13.36,8.43,6.63,4.52和4.27%。虽然(20:4-20:4-20:4),(20:4-24:0-20:4),(20:3-20:4-16:0),(20:4-16: 0-20:4)和(18:1-16:0-20:3)是第二种样品中的主要物种,相对含量为11.47,8.65,7.84,6.34和4.31%。此外,花生酸,木质酸和棕榈酸是ARA-SCO样品中的主要脂肪酸。这是第一个报告ARA-SCO标签分数的广泛表征的第一项工作,这可能代表了结构化脂质和脂质族菌领域的进一步研究和应用的关键。

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  • 作者单位

    Jiangnan Univ Sch Food Sci &

    Technol Natl Engn Res Ctr Funct Fo Collaborat Innovat Ctr Food Safety &

    Qual Control Natl Engn Lab Cereal Fermentat Technol State Key 1800 Lihu Rd Wuxi 214122 Jiangsu Peoples R China;

    Jiangnan Univ Sch Food Sci &

    Technol Natl Engn Res Ctr Funct Fo Collaborat Innovat Ctr Food Safety &

    Qual Control Natl Engn Lab Cereal Fermentat Technol State Key 1800 Lihu Rd Wuxi 214122 Jiangsu Peoples R China;

    Jiangnan Univ Sch Food Sci &

    Technol Natl Engn Res Ctr Funct Fo Collaborat Innovat Ctr Food Safety &

    Qual Control Natl Engn Lab Cereal Fermentat Technol State Key 1800 Lihu Rd Wuxi 214122 Jiangsu Peoples R China;

    Jiangnan Univ Sch Food Sci &

    Technol Natl Engn Res Ctr Funct Fo Collaborat Innovat Ctr Food Safety &

    Qual Control Natl Engn Lab Cereal Fermentat Technol State Key 1800 Lihu Rd Wuxi 214122 Jiangsu Peoples R China;

    Jiangnan Univ Sch Food Sci &

    Technol Natl Engn Res Ctr Funct Fo Collaborat Innovat Ctr Food Safety &

    Qual Control Natl Engn Lab Cereal Fermentat Technol State Key 1800 Lihu Rd Wuxi 214122 Jiangsu Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 食品工业;
  • 关键词

    Food composition; Food analysis; Single-cell oil; Arachidonic acid; Triacylglycerols; Fatty acid composition; Mortierella alpina; Polyunsaturated fatty acids; UPLC-Q-TOF-MS;

    机译:食品分析;单细胞油;花生酸;三酰基甘油;脂肪酸组成;紫龙酸性组成;多不饱和脂肪酸;UPLC-Q-TOF-MS;UPLC-Q-TOF-MS;

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