Enhanced HPLC-DAD Method for Fast Determination of Quercetin-3-O-beta-D-Glucoside in Extracts and Polyherbal Formulations Containing Azadirachta indica-Optimization and Validation

摘要

Azadirachta indica has been used for its medicinal properties since time immemorial. Herbal medicines which are prepared using this medicinal tree are utilized to treat various diseases and disorders. No reports are available for marker-based standardization of these herbal medicines prepared from leaves of A. indica. Also existing HPLC methods for determination quercetin-3-O-beta-D- glucoside are time consuming. There is an obvious need for development of new HPLC method for quantification of quercetin-3-O-beta-D-glucoside which is fast enough to carry out analysis in stipulated time period. This article deals with the development, optimization and validation of fast HPLC-DAD method for the determination of quercetin-3-O-beta-D-glucoside in extracts containing A. indica for its successive application for marker-based standardization of herbal formulations containing A. indica. The retention time of quercetin-3-O-beta-D-glucoside was 11.213 min. The method was found to be linear in the range of 4.0-60 mu g mL(-1). Limit of detection and limit of quantitation of the proposed method were found to be 1.33 and 4.0 mu g mL(-1), respectively. The mean recoveries were found to be within 93.53-103.75%. The method can be used as quality control tool for routine analysis of herbal extracts and formulations containing A. indicia.