l l ‐arginine protects against oxidative damage induced by T‐2 toxin in mouse Leydig cells

摘要

Abstract To explore the protective mechanism of l ‐arginine against T‐2 toxin–induced oxidative damage in mouse Leydig cells, Leydig cells were isolated and cultured with control, T‐2 toxin (10?nM), l ‐arginine (0.25, 0.5, and 1.0?mM), and T‐2 toxin (10?nM T‐2 toxin) with l ‐arginine (0.25, 0.5, or 1.0?mM) for 24?hours. Cells and supernatants were harvested to examine cell viability, activities, and messenger RNA (mRNA) expression of glutathione peroxidase (GSH‐Px), superoxide dismutase (SOD), and catalase (CAT), malondialdehyde (MDA) content, and DNA damage. Results showed that T‐2 toxin significantly reduced cell viability, improved MDA content and DNA damage, and decreased activities and mRNA expression of GSH‐Px, SOD, and CAT. However, l ‐arginine?reduced T‐2 toxin–induced oxidative damage and tended to maintain normal levels. Furthermore, l ‐arginine upregulated mRNA expressions of GSH‐Px, SOD, and CAT. Collectively, l ‐arginine, due to its antioxidative ability, could ameliorate T‐2 toxin–induced cytotoxicities in mouse Leydig cells by regulating oxidative stress.