Basic principles for developing real-time PCR methods used in food analysis: A review

摘要

Background: The increased interest in global food fraud has led to the development of a number of advanced methods, among which real-time polymerase chain reaction (PCR) currently plays an integral role in food authentication. However, the lack of standard parameters for the development and validation of real-time PCR methods hampers their utilization across different laboratories and conditions, leading to inconsistent results. Scope and approach: This review summarizes and assesses different methods presented throughout a large number of scientific papers, including DNA extraction, primer design, and quantification (or qualification) as well as parameters for the development and validation of real-time PCR methods in food analysis.