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Paratuberculosis in red deer (Cervus elaphus hippelaphus) in the WesternAlps

机译:在红鹿(鹿Eleaphus hippelaphus)的伞菌化在西方行程

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During the hunting seasons 1995-96 to 1997-98, 19 red deer from the Upper Susa Valley (Cottian Alps) were examined for paratuberculosis (Johne's disease). Specific DNA amplification on mesenteric lymph nodes detected Mycobacterium avium paratuberculosis in 17 animals. Ten of these red deer were tested for serum antibodies by the AGID and ELISA tests, nine being negative. Three isolates from infected deer were genetically characterized by an arbitrarily primed polymerase chain reaction, and showed similar genetic polymorphism to that of bovine strains isolated in different Italian areas. The study showed that paratuberculosis is present in red deer of the Upper Susa Valley and that serological tests are not an efficient means for monitoring this infection.
机译:在1995-96至1997-98至1997-98期间,研究了来自上苏萨谷(棉花阿尔卑斯山)的19块红鹿,用于治疗(约翰氏症)。 肠系膜淋巴结的特异性DNA扩增检测到17只动物中的分枝杆菌抗血管刺激。 通过琼脂和ELISA试验测试血清抗体中的十种,九个是阴性的。 来自受感染鹿的三个分离物在基因表征是由任意引发的聚合酶链反应的特征,并显示出与不同意大利区域中分离的牛菌株的类似遗传多态性。 该研究表明,对苏萨谷的红鹿的副刺激存在,并且血清学试验不是监测这种感染的有效手段。

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