Cytoprotection Activity Evaluation of (E)-3-(3,4-Dichlorophenyl)-1-(3,4-Dihydroxyphenyl)- Prop-2-En-1-One as a New Antioxidant Against H2O2-Induced Oxidative Damage in PC12 Cells

摘要

Scavenging reactive oxygen species (ROS) by antioxidants is an important therapy to neurodegenerative diseases. In this work, a new chalcone compound (E)-3-(3,4 -dichlorophenyl)-1-(3,4-dihydroxyphenyl)-prop-2-en-1-one (A1) with potent antioxidant activity in PC12 cells was found. The MTT assay showed that A1 at 10 mu M had no apparent cytotoxicity in PC12 cells. DPPH assay exhibited A1 could scavenge ROS directly. Treatment with A1 could elevate the population of viable cells in a dose-dependent manner. Furthermore, A1 protected PC12 cells against H2O2-induced apoptosis. At the molecular level, A1 conferred cytoprotection against oxidative damage in PC12 cells via up-regulation of the expression of HO-1 and GCLC proteins. These findings suggest compound A1 to be a promising antioxidant for the treatment oxidative stress-related diseases.