Further evidence for the role of WNT 10A, WNT 10B WNT WNT 10A, WNT WNT 10B and GREM 2 GREM GREM 2 as candidate genes for isolated tooth agenesis

摘要

Structured Abstract Objective To investigate the association of single nucleotide variants in the candidate genes WNT 10A, WNT 10B and GREM 2 with isolated tooth agenesis. Setting and sample population A total of 435 Caucasian individuals (88 cases with isolated tooth agenesis and 347 unrelated controls) were ascertained at the University of Texas Health Science Center at Houston School of Dentistry. Clinical and radiographic examination by orthodontists confirmed the diagnosis of tooth agenesis. Genetic evaluation excluded syndromic forms of tooth agenesis. Materials and methods Saliva samples were collected as source of genomic DNA . Fourteen variants in/nearby WNT 10A , WNT 10B and GREM 2 were genotyped to test for association with tooth agenesis. Genotyping was performed using TaqMan chemistry in a real‐time polymerase chain reaction assay. Allelic and haplotype frequencies were compared among cases and controls using chi‐square tests as implemented in PLINK v.1.06. Bonferroni correction was used and P ?≤?0.004 indicates statistical differences between groups. Results Significant associations were found between individual SNP s and SNP combinations in WNT 10A, WNT 10B and GREM 2 SNP s with isolated tooth agenesis ( P ??0.004). Conclusion Our findings further support a role for variants in WNT 10A, WNT 10B and GREM 2 genes in the aetiology of isolated tooth agenesis. Functional studies are necessary to investigate the biological effects of these gene variants in tooth agenesis phenotypes.