首页> 外文期刊>Oral and maxillofacial surgery. >The expression of melanoma-associated antigen A (MAGE-A) in oral squamous cell carcinoma: an evaluation of the significance for tumor prognosis
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The expression of melanoma-associated antigen A (MAGE-A) in oral squamous cell carcinoma: an evaluation of the significance for tumor prognosis

机译:口腔鳞状细胞癌中黑色素瘤相关抗原A(MAGE-A)的表达:对肿瘤预后的意义评估

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Objectives Melanoma-associated antigens A had been detected repeatedly in oral squamous cell carcinoma, but not in healthy mucosa. Additionally, patients with MAGE-A expressing cancers are regarded to have a worse survival prognosis, so that MAGE-A are supposed to be part of carcinogenesis. Which role these antigens fulfill within OSCC is still, up today, largely unknown. This study examines the hypothesis that MAGE-A is being produced in OSCC but not in mucosa tissue and if MAGE-A has any correlation to clinical patient's parameters like tumor size, lymph node metastasis, distant metastasis, overall survival, and recurrence. Materials and methods For this purpose, 50 tumor samples and 39 mucosa samples were analyzed by means of PCR and immunohistochemical staining with the antibody 6C1. Results Forty of 41 stained tumor samples showed a positive antibody reaction with a maximum staining rate of 53%. Sixteen mucosa samples showed a mild positive reaction. The PCR revealed a linear expression pattern of MAGE-A in which the genes are proportionally expressed in OSCC. We did not find any relationship between MAGE-A and tumor size, overall survival, or recurrence. There was also no connection between MAGE-A and tumor parameters Hif-1 and LDH. Their expression was detected tendentially in tumors with higher staging, advanced lymph node metastasis, and rising age of the patients. The genes MAGE-A3+6 and MAGE-A4 had a statistically significant correlation with lymph node metastasis (p = 0.007 and p = 0.004). Patients got distant metastasis and influence of MAGE-A on metastatic behavior could not be verified. The genes MAGE-A3 and -A4 are consequently qualified as tumor markers in the field of diagnosis and follow-up of OSCC. Conclusions and clinical relevance Two genes have great potential as target proteins in immunotherapy. The genes MAGE-A3+6 and MAGE-A4 had a statistically significant correlation with lymph node metastasis.
机译:目标黑色素瘤相关抗原A在口腔鳞状细胞癌中反复检测到,但不在健康的粘膜中进行检测。此外,患有MAGE-A表达癌症的患者被认为具有更差的存活预后,因此法师A应该是致癌物发生的一部分。今天仍然在OSCC中满足这些抗原在OSCC中仍然存在的作用,这在很大程度上是未知数。该研究检查了MAGE-A在OSCC中产生但不在粘膜组织中产生的假设,并且如果MAGE-A与肿瘤大小,淋巴结转移,远处转移,整体存活和复发有任何相关性与临床患者参数的相关性。通过PCR和用抗体6C1分析50种肿瘤样品和39粘膜样品的材料和方法。结果41个染色肿瘤样品的41个阳性抗体反应,最大染色率为53%。 16张粘膜样品显示出温和的阳性反应。 PCR揭示了MAGE-A的线性表达模式,其中基因在OSCC下比例地表达。我们没有发现Mage-A和肿瘤大小之间的任何关系,整体存活或复发。 MAGE-A和肿瘤参数HIF-1和LDH之间也没有连接。它们的表达被倾向于具有较高分期,晚期淋巴结转移和患者的上升时代的肿瘤中的肿瘤。基因MAGE-A3 + 6和MAGE-A4与淋巴结转移具有统计学显着的相关性(P = 0.007和P = 0.004)。患者远处转移,并且无法验证MAGE-A对转移行为的影响。因此,基因MAGE-A3和-A4因此被称为OSCC诊断和随访领域的肿瘤标志物。结论和临床相关性两种基因具有巨大的免疫疗法靶蛋白。基因MAGE-A3 + 6和MAGE-A4与淋巴结转移具有统计学显着的相关性。

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