Immunity Theory-Based High-Specific Monoclonal Antibody Preparation and Application of Fumonisin B-1

摘要

Antibody preparation is the most important step in immunoassays. According to immunity theory (IT) "Antibody tends to recognize the furthest distance of side groups in the coupling league,"; immunogen must be well designed for high-specific antibody production for high-specific antibody preparation. In this research, a novel fumonisin B-1 immunogen was synthesized in which protein was conjugated at C2 position to expose the distant two type-feature groups of side chains at C14 and C15 position. After immunization, a high-specific anti-FB1 monoclonal antibody (mAb) was produced with negligible CR value to analogues, including the most structure-related FB2 and FB3. Based on this mAb, a specific ELISA method was developed and validated for FB1 detection in rice with limit of detection at 0.0339 ng kg(-1). In real-life samples, 20% were detected positive in 10 samples, with FB1 concentrations at 84 and 20 ng kg(-1), respectively.