OPTIMIZING SPORULATION OF Pseudocercospora griseola IN VITRO

摘要

Angular leaf spot of common bean (ALS), caused by fungus Pseudocercospora griseola (Sacc.) Crous & U. Braun, is one of the major diseases that affect the crop (Singh & Schwartz, 2010). Development of resistant cultivars is one of main objectives of common bean breeding programs and success in selection of lines and progenies depends on accurate assessments of ALS severity. High conidia production in vitro is difficult and makes the use of artificial inoculation in common bean plants unfeasible (Sanglard et al., 2009). Development of efficient methodologies in conidia production to artificial inoculation has been reported in literature, however, average time to obtain enough sporulation is around 30 to 45 days (Sanglard et al., 2009; Silveira 1967).In this study we present a new protocol for sporulation of P. griseola and compare its efficiency with other methods already described in literature.