Method of facilitating mass production and sporulation of arbuscular mycorrhizal fungi aseptic in vitro

摘要

Provided is a method for the in vitro aseptic mass production and sporulation of arbuscular mycorrhizal fungi (AMF). The method comprises providing symbiotic root organs with AMF propagules and spores after the root organs are inoculated with AMF inoculums in advance to form AMF propagules in the root organs and cultivating them in an aseptic container, and then providing the whole symbiotic root organs and AMF propagules with liquid medium for a temporary contact between the liquid medium and the root organ with AMF propagules in order to cultivate AMF, then removing the liquid medium from the symbiotic root organs and arbuscular mycorrhizal fungal propagules and finally repeating former two steps periodically to facilitate the mass production and sporulation of the arbuscular mycorrhizal fungi.