Optimization of uptake and transport of gold nanoparticles in two-dimensional and three-dimensional in-vitro cell models

摘要

High atomic number nanomaterials have been explored as a tool for improving cancer therapeutics. Goldnanoparticles are a system that has been introduced as they can act as eu000bective radiation dose enhancers andanticancer drug carries. Gold nanoparticles have unique physiochemical properties that allow them to be probedin cells using techniques such as scanning electron microscopy and hyper spectral imaging. Optimization of goldnanoparticle uptake into 3D in-vitro models is essential to optimizing future cancer therapeutic applicationsand bridging the gap between in-vitro and in-vivo tumour environments. The uptake of functionalized goldnanoparticles into 2D monolayer and 3D spheroid cell models was tested. Functionalization of the GNPs wasconrmed by use of dynamic light scattering, UV-Visible light spectroscopy, the Zeta potential, and imagedwith a scanning electron microscope and hyper spectral imaging. These ndings suggest that both the size andfunctionalization of the gold nanoparticles should be considered in future 3D in-vitro studies.