Effect of adriblastin on viability, cell cycle and apoptosis in B16 and cloudman s91 mouse melanoma cells in vitro.

摘要

Recently, the oncologists have been increasingly interested in apoptosis. which is morphologically. biochemically and topographically different from necrosis. Apoptosis is probably a key point to understand the resistance of malignant cells to cytotoxic drug. The aim of this study was to determine the influence of adriblastin on morphology and kinetics of cell proliferation and evaluation of apoptosis and necrosis in B16 and Cl S91 mouse melanoma cells in vitro. Adriblastin induced morphological changes indicating occurrence of apoptosis and necrosis in B16 and Cl S91 mouse melanoma cells. The number of viable B16 and Cl S91 cells was estimated by flow cytometry analysis. Apoptotic cells were detected using the annexin V-FITC test. DNA content, after staining cells with propidium iodide, was analysed by flow cytometry. B16 cell line was more sensitive to adriblastin treatment than the CIS91 cell line. The adriblastin EC50, value estimated for the B16 line was 2.4 microM. while for Cl S91 line was 4.0 microM. It was revealed that about 59% Cl S91 cells and 65% B16 cells had died in the apoptotic way after adriblastin addition at EC50, concentration to B16 and Cl S91 cultures. Adriblastin induced cell arrest in G1 and S cell cycle phase in both B16 and Cl S91 cell lines. CONCLUSION: the new solutions to determinate cell chemosensitivity should be introduced into clinical practice.