Increased Yield of Small DNA Fragments Purified by Silica Binding

摘要

Removal of con laminating factors from DNA is an important step in many laboratory applications. For example, primers, excess dNTPs and sails left over after (he polymerase chain reaction (PCR) can interfere with subsequent steps in many procedures and must be removed. Since such a purification slep may have to be performed more than once in a given procedure, it is important to use a method that is simple, rapid and minimizes DNA loss, A technique that has been found useful in this laboratory is the Geneclean~(R) II method from Bio 101 (La Jolla, CA, USA). This silica-based (Glassmilk~(R); Bio 101) process allows binding of DNA so contaminating molecules may be washed away, and the purified DNA can be cluted in water or buffer.