humanized ANTIBODY AND THE ANTIGEN-BINDING FRAGMENT (FAB), COMING ARE CONNECTED THE VERSATILE OF INTERFERON- GAMMA PERSON, THE FRAGMENTS OF DNA, WHICH CODE THE ANTIBODY CONTAINER AND THE ANTIGEN-BINDING FRAGMENT, THE CELL, TRANSFORMABLE BY FRAGMENT DNA, AND METHOD OF OBTAINING ANTIBODY CONTAINER AND ANTIGEN-BINDING FRAGMENT

摘要

FIELD: medicine, pharmaceutics.;SUBSTANCE: invention relates to field of immunology and biotechnology. Described are: humanised antibody and its antigen-binding fragment (Fab), which selectively bind human IFN-γ and contain variable site of heavy chain (VH) and variable site of light chain (VL), where VH and VL have sequences of amino acids respectively SEQ ID NO:1 and 2, presented in description. Also disclosed are fragments of DNA, which code said antibody and its Fab-fragment; plasmid DNA for expression of said specific proteins; and modified cells of bacteria and eukaryotes, which contain said plasmid DAN and intended for expression of said antibody and its Fab-fragment. Claimed are methods of obtaining said antibody and its Fab-fragment, which include cultivation of said modified cells in nutritional medium and isolation of antibody and its Fab-fragment by claimed invention from cultural liquid.;EFFECT: invention makes it possible to obtain humanised antibody or its Fab-fragment, binding with IFN-γ with kD 4,6 and IC₅₀ not less than 1,5 nM in test on cells U937, with preservation of affinity of initial mouse monoclonal antibody.;13 cl, 3 dwg, 1 tbl, 8 ex