Engineering and expression of a recombinant fusion protein possessing fibroblast growth factor-2 and fibronectin fragment

摘要

There is a synergistic effect between fibronectin and fibroblast growth factor 2(FGF-2)on osteoblast cell adhesion through an extracellular,signal-regulated kinase pathway.Here we describe the engineering of a fusion protein containing fibronectin fragment(FNIII9-10)connected to the COOH-terminus of FGF-2.Purified FGF2-FNIII9-10 fusion protein exhibited a significant increase of cell adhesion and proliferation of MG63 cells compared with FNIII9-10 alone(p < 0.05).