Enzymatic transformation of ginsenosides Re, Rg1, and Rf to ginsenosides Rg2 and aglycon PPT by using beta-glucosidase from Thermotoga neapolitana

摘要

ObjectivesTo enzymatically transform protopanaxatriol by using -glucosidase from Thermotoga neapolitana (T. neapolitana) DSM 4359.ResultsRecombinant -glucosidase was purified, which molecular weight was about 79.5kDa. High levels of ginsenoside were obtained using the follow reaction conditions: 2mgml(-1) ginsenoside, 25 U ml(-1) enzyme, 85 degrees C, and pH 5.0. -glucosidase converted ginsenoside Re to Rg2, Rf and Rg1 to APPT completely after 3h under the given conditions, respectively. The enzyme created 1.66mgml(-1) Rg2 from Re with 553mgl(-1) h(-1), 0.85mgml(-1), and 1.01mgml(-1) APPT from Rg1 and Rf with 283 and 316mgl(-1) h(-1) APPT.Conclusions-glucosidase could be useful for the high-yield, rapid, and low-cost preparation of ginsenoside Rg2 from Re, and APPT from the ginsenosides Rg1 and Rf.