Expression of a novel manganese peroxidase from Cerrena unicolor BBP6 in Pichia pastoris and its application in dye decolorization and PAH degradation

摘要

Manganese peroxidase (MnP), which is an extracellular glycosylated heme protein produced by various basi-diomycetous fungi, shows attractive potential in various biotechnological applications. A novel MnP gene, mnp3, from white-rot fungus Cerrena unicolor BBP6 was cloned and expressed in Pichia pastoris with a MnP activity of 154.5 U/l. The recombinant enzyme, rMnP3-BBP6, had strong decolorizing activity on a variety of dyes and it was efficient in denim bleaching. In addition, it was able to degrade fluorene and phenanthrene effectively. The results of this research demonstrated that rMnP3-BBP6 had promising biotechnological application potential in textile industries and polycyclic aromatic hydrocarbon bioremediation.