Integrated multi-spectroscopic and molecular modelling techniques to probe the interaction mechanism between salvianolic acid A and alpha-glucosidase

摘要

alpha-Glucosidase (AG) is an important drug target for the treatment of type 2 diabetes mellitus in humans due to the potential effect of down regulating glucose absorption in patients. In our previous study, salvianolic acid A (SAA) was found to exhibit potent AG inhibitory activity, whereas the interaction mechanism was still ambiguous. Herein, the interaction mechanism of SAA and AG was investigated by multi-spectroscopic methods along with molecular docking. As a result, it was found that SAA reversibly inhibited AG in a competitive manner with IC50 of 16.44 +/- 0.18 mu M, and the inhibition belonged to a multi-phase kinetics process with a first-order reaction. The intrinsic fluorescence of AG could be strongly quenched by SAA through a static quenching mechanism. The negative Gibbs free energy change and positive values of enthalpy and entropy change revealed that the binding of SAA to AG was spontaneous and dominated mainly by hydrophobic interactions, and only a single binding site was determined for them. Analysis of synchronous fluorescence, ANS-binding fluorescence, circular dichroism and Fourier transform infrared spectra suggested that the binding of SAA to AG induced rearrangement and conformational changes of the enzyme. Besides, further molecular modelling validated that SAA could bind to the active domain and prevent the entrance of substrate, resulting in the inhibition of AG activity. These findings provide new insights into understanding the interaction mechanism of SAA on AG. (c) 2019 Elsevier B.V. All rights reserved.