MicroRNA-379 inhibits cell proliferation and invasion in glioma via targeting metadherin and regulating PTEN/AKT pathway

摘要

Numerous microRNAs (miRNAs) are aberrantly expressed in glioma, and implicated in glioma occurrence and development. Therefore, the development of miRNAs as potential therapeutic targets for the treatment of patients with glioma has been proposed. miR-379 has been shown to be aberrantly expressed in the progression of malignant tumours. However, the expression, biological functions and mechanism of miR-379 in glioma are yet to be fully understood. Hence, the present study aimed to detect miR-379 expression, investigate its functional relevance and explore its associated molecular mechanism in glioma. In this study, miR-379 expression was significantly downregulated in glioma tissues and cell lines. Enforced miR-379 expression markedly suppressed the cell proliferation and invasion of glioma. Metadherin (MTDH) was identified as a direct target of miR-379 in glioma. The miR-379 expression and MTDH mRNA levels exhibited an inverse association in glioma tissues. The restoration of the MTDH expression partially rescued the inhibitory effects of miR-379 overexpression on glioma cell proliferation and invasion, and the upregulation of miR-379 inhibited the activation of phosphatase and tensin homolog (PTEN)/AKT serine/threonine kinase (AKT) signaling pathway. Overall, these findings demonstrated that miR-379 may play tumour-suppressing roles in glioma through downregulation of MTDH and regulation of the PTEN/AKT signaling pathway, suggesting that miR-379 might be a possible target for the treatment of patients with this malignancy.