Microfabrication of patterns of adherent marine bacterium phaeobacter inhibens using soft lithography and scanning probe lithography

摘要

Two lithographic approaches have been explored for the microfabrication of cellular patterns based on the attachment of marine bacterium Phaeobacter inhibens strain T5. Strain T5 produces a new antibiotic that makes this bacterium potentially interesting for the pharmaceutical market and as a probiotic organism in aquacultures and in. controlling biofouling. The microcontact printing (μCP) method is based on the micropatterning of self-assembled monolayers (SAMs) terminated with adhesive end groups such as CH_3 and COOH and nonadhesive groups (e.g., short oligomers of ethylene glycol. (OEG)) to form micropattemed substrates for the adhesion of strain T5. The scanning probe lithographic method is based on the surface modification of OEG SAM by using a microelectrode, the probe of a scanning electrochemical microscope (SECM). Oxidizing agents (e.g., Br_2) were electrogenerated in situ at the microelectrodes from Br- in aqueous solution to remove OEG SAMs locally, which allows the subsequent adsorption of bacteria. Various micropatterns of bacteria could be formed in situ on the substrate without a prefabricated template. The fabricated cellular patterns may be applied to a variety of marine biological studies that require the analysis of biofilm formation, cell-cell and. cell-surface interactions, and cell-based biosensors and bioelectronics.