Simultaneous Determination of Four Neuritogenic Compounds in Gentiana rigescens from Different Regions by Ultra-Performance Liquid Chromatography Tandem Mass Spectrometry

摘要

The roots and rhizomes of Gentiana rigescens are the original materials of Chinese traditional medicine "LongDan" which is used for treatment of hepatitis and cholecystitis for over a thousand years. In this study, the concentrations of four gentisides (gentiside A, B, J and K) were determined by UPLC-MS/MS in Gentiana rigescens from 2 different regions (Yunxian and Yongde) of southwest Yunnan in China. The maximum concentration of gentiside A, B, J and K were found in S5, S12, S5 and S3, respectively. Hierarchical clustering analysis showed the maximum total concentration of gentisides was in group IV, which indicated S5 could be regarded as optimizing original materials of G. rigescens. The cultivated samples collected from Yunxian showed the higher contents of gentisides. These results would provide information for sustainable utilization of G. rigescens.