Determination of vitamin E and carotenoid pigments by high performance liquid chromatography in shell of Chionoecetes opilio

摘要

This study reports the optimization of a method for the determination of vitamin E and carotenoids in shells of Chionoecetes opilio samples by online HPLC coupled with UV-vis and fluorescence detectors. The carotenoids were determined with diode-array detector (λ 450 nm) and vitamin E with fluorescence detection (λ_(ex) 288, λ_(em) 331 nm). Two extractions methods were compared, saponification followed by an extraction step and a simple extraction with acetone. The last one was selected because allows to determine all compounds. Linearity, precisions and recoveries achieved for all compounds were satisfactory. Mean concentrations (mg per 100 g dry weight) were; 23.3 for vitamin E, 9.49 for astaxan-thin and 0.2 mg for β-carotene.